Coding

Part:BBa_K2617017:Design

Designed by: Changyu Li   Group: iGEM18_UESTC-China   (2018-09-30)


J23100-RBS-pelB-5D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 115
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To get a good expression of this gene in E. coli, we did codon optimization before DNA synthesis.


Source

The source of the part will be its sequence which was retrieved from GenBank.

References